Context transcription factors establish cooperative environments and mediate enhancer communication.

ABSTRACT: Many enhancers control gene expression by assembling regulatory factor clusters, also referred to as condensates. This process is vital for facilitating enhancer communication and establishing cellular identity. However, how DNA sequence and transcription factor (TF) binding instruct the formation of high regulatory factor environments remains poorly understood. Here we developed a new approach leveraging enhancer-centric chromatin accessibility quantitative trait loci (caQTLs) to nominate regulatory factor clusters genome-wide. By analyzing TF-binding signatures within the context of caQTLs and comparing episomal versus endogenous enhancer activities, we discovered a class of regulators, 'context-only' TFs, that amplify the activity of cell type-specific caQTL-binding TFs, that is, 'conte

SUBMITTER: Kribelbauer-Swietek JF 

PROVIDER: S-EPMC11525195 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature