CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells.

ABSTRACT: Ageing impairs the ability of neural stem cells (NSCs) to transition from quiescence to proliferation in the adult mammalian brain. Functional decline of NSCs results in the decreased production of new neurons and defective regeneration following injury during ageing^1-4. Several genetic interventions have been found to ameliorate old brain function^5-8, but systematic functional testing of genes in old NSCs-and more generally in old cells-has not been done. Here we develop in vitro and in vivo high-throughput CRISPR-Cas9 screening platforms to systematically uncover gene knockouts that boost NSC activation in old mice. Our genome-wide screens in primary cultures of young and old NSCs uncovered more than 300 gene knockouts that specifically restore the activation of old

SUBMITTER: Ruetz TJ 

PROVIDER: S-EPMC11525198 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature