Protocol for protein modification using oxalyl thioester-mediated chemoselective ligation.

ABSTRACT: The development of fast ligation chemistries for the site-specific modification of proteins has become a major focus in chemical biology. We describe steps for preparing an oxalyl thioester precursor in the form of an N-oxalyl perhydro-1,2,5-dithiazepine handle, i.e., the ^oxoSEA group, and incorporating it into a peptide modifier using solid phase peptide synthesis. We then detail procedures for its application for the modification of an N-terminal Cys-containing B1 domain of the streptococcal G protein using the native chemical ligation. For complete details on the use and execution of this protocol, please refer to Snella et al.¹.

SUBMITTER: Terzani F 

PROVIDER: S-EPMC11525222 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature