Multiplexed Assay for Small-Molecule Quantification via Photo-cross-linking of Structure Switching Aptamers.

ABSTRACT: There is an unmet need for molecular detection assays that enable the multiplexed quantification of small-molecule analytes. We present xPlex, an assay that combines aptamer switches with ultraviolet-cross-linkable complementary strands to record target-binding events. When the aptamer's small-molecule target is present, the cross-linkable strand is displaced, enabling PCR amplification and detection of the relevant aptamer. In the absence of that target, the aptamer is readily cross-linked to the strand, preventing amplification from happening. The resulting aptamer-specific amplicons can be detected and quantified in a multiplexed fashion using high-throughput sequencing. We demonstrate quantitative performance for a pair of small-molecule analytes, dopamine and glucose, and show that th

SUBMITTER: Newman SS 

PROVIDER: S-EPMC11525510 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature