Deciphering craniopharyngioma subtypes: Single-cell analysis of tumor microenvironment and immune networks.

ABSTRACT: Craniopharyngiomas, including adamantinomatous (ACP) and squamous papillary (PCP) types, are challenging to treat because of their proximity to crucial pituitary structures. This study aimed to characterize the cellular composition, tumor tissue diversity, and cell-cell interactions in ACPs and PCPs using single-cell RNA sequencing. Single-cell clustering revealed diverse cell types, further classified into developing epithelial, calcification, and immune response for ACP and developing epithelial, cell cycle, and immune response for PCP, based on gene expression patterns. Subclustering revealed the enrichment of classical M1 and M2 macrophages in ACP and PCP, respectively, with high expression of pro-inflammatory markers in classical M1 macrophages. The classical M1 and M2 macrophage rati

SUBMITTER: Matsuda T 

PROVIDER: S-EPMC11525618 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature