Encoding extracellular modification of artificial cell membranes using engineered self-translocating proteins.

ABSTRACT: The development of artificial cells has led to fundamental insights into the functional processes of living cells while simultaneously paving the way for transformative applications in biotechnology and medicine. A common method of generating artificial cells is to encapsulate protein expression systems within lipid vesicles. However, to communicate with the external environment, protein translocation across lipid membranes must take place. In living cells, protein transport across membranes is achieved with the aid of complex translocase systems which are difficult to reconstitute into artificial cells. Thus, there is need for simple mechanisms by which proteins can be encoded and expressed inside synthetic compartments yet still be externally displayed. Here we present a genetically enco

SUBMITTER: Harjung A 

PROVIDER: S-EPMC11526174 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature