A gradient model of renal ischemia reperfusion injury to investigate renal interstitial fibrosis.

ABSTRACT: Background: The progression from acute kidney injury to chronic kidney disease poses a significant health challenge. Nonetheless, a constraint in existing animal models of renal ischemia/reperfusion (I/R) injury is the necessity for a severe injury, almost reaching a life-threatening level, to trigger the subsequent onset of renal fibrosis. Hence, we explored an adapted gradient approach to induce I/R injury, aiming to promote the progression of renal fibrosis while preserving the overall normal functioning of the kidney. Methods: In each group, 6-8 male C57BL/6 mice were used for model construction, with all undergoing sodium pentobarbital anesthesia and left kidney removal. Subsequently, a silk thread was passed beneath the lower renal branch, elevating the right kidney und

SUBMITTER: Yang F 

PROVIDER: S-EPMC11526246 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature