Point-Spread Function Deformations Unlock 3D Localization Microscopy on Spherical Nanoparticles.

ABSTRACT: Nanoparticles (NPs) have proven their applicability in biosensing, drug delivery, and photothermal therapy, but their performance depends critically on the distribution and number of functional groups on their surface. When studying surface functionalization using super-resolution microscopy, the NP modifies the fluorophore's point-spread function (PSF). This leads to systematic mislocalizations in conventional analyses employing Gaussian PSFs. Here, we address this shortcoming by deriving the analytical PSF model for a fluorophore near a spherical NP. Its calculation is four orders of magnitude faster than numerical approaches and thus feasible for direct use in localization algorithms. We fit this model to individual 2D images from DNA-PAINT experiments on DNA-coated gold NPs and demonst

SUBMITTER: Huijben TAPM 

PROVIDER: S-EPMC11526427 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature