Sequencing the orthologs of human autosomal forensic short tandem repeats provides individual- and species-level identification in African great apes.

ABSTRACT:

Background

Great apes are a global conservation concern, with anthropogenic pressures threatening their survival. Genetic analysis can be used to assess the effects of reduced population sizes and the effectiveness of conservation measures. In humans, autosomal short tandem repeats (aSTRs) are widely used in population genetics and for forensic individual identification and kinship testing. Traditionally, genotyping is length-based via capillary electrophoresis (CE), but there is an increasing move to direct analysis by massively parallel sequencing (MPS). An example is the ForenSeq DNA Signature Prep Kit, which amplifies multiple loci including 27 aSTRs, prior to sequencing via Illumina technology. Here we assess the applicability of this human-based kit in African great apes. We

SUBMITTER: Fedele E 

PROVIDER: S-EPMC11526555 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature