Quantitative micro-CT-derived biomarkers elucidate age-related lung fibrosis in elder mice.

ABSTRACT:

Background

Idiopathic Pulmonary Fibrosis (IPF), prevalently affecting individuals over 60 years of age, has been mainly studied in young mouse models. The limited efficacy of current treatments underscores the need for animal models that better mimic an aged patient population. We addressed this by inducing pulmonary fibrosis in aged mice, using longitudinal micro-CT imaging as primary readout, with special attention to animal welfare.

Methods

A double bleomycin dose was administered to 18-24 months-old male C57Bl/6j mice to induce pulmonary fibrosis. Bleomycin dosage was reduced to as low as 75% compared to that commonly administered to young (8-12 weeks-old) mice, resulting in long-term lung fibrosis without mortality, complying with animal welfare guidelines. After fibros

SUBMITTER: Buseghin D 

PROVIDER: S-EPMC11526612 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature