Monosynaptic tracing: a step-by-step protocol.

ABSTRACT: Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons before subsequent injection of the rabies virus. Here we present a step-by-step protocol for performing such experiments using first-generation (ΔG) rabies viral vectors.

SUBMITTER: Lavin TK 

PROVIDER: S-EPMC11526806 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature