A rapid, facile, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies.

ABSTRACT: Ribosomes are macromolecular RNA-protein complexes that constitute the central machinery responsible for protein synthesis and quality control in the cell. Ribosomes also serve as a hub for multiple non-ribosomal proteins and RNAs that control protein synthesis. However, the purification of ribosomes and associated factors for functional and structural studies requires a large amount of starting biological material and a tedious workflow. Current methods are challenging as they combine ultracentrifugation, the use of sucrose cushions or gradients, expensive equipment, and multiple hours to days of work. Here, we present a rapid, facile, and cost-effective method to isolate ribosomes from in vivo or in vitro samples for functional and structural studies using single-step enric

SUBMITTER: Erath J 

PROVIDER: S-EPMC11526893 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature