Exploring new dimensions of immune cell biology in <i>Anopheles gambiae</i> through genetic immunophenotyping.

ABSTRACT: Mosquito immune cells, or hemocytes, are integral components of the innate immune responses that define vector competence. However, the lack of genetic resources has limited their characterization and our understanding of their functional roles in immune signaling. To overcome these challenges, we engineered transgenic Anopheles gambiae that express fluorescent proteins under the control of candidate hemocyte promoters. Following the characterization of five transgenic constructs through gene expression and microscopy-based approaches, we examine mosquito immune cell populations by leveraging advanced spectral imaging flow cytometry. Our results comprehensively map the composition of mosquito hemocytes, classifying them into twelve distinct populations based on size, granularity, pl

SUBMITTER: Samantsidis GR 

PROVIDER: S-EPMC11526922 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature