Genetically-Encoded Fluorescence Barcodes for Single-Cell Analysis.

ABSTRACT: Genetically-encoded, single-cell barcodes are broadly useful for experimental tasks such as lineage tracing or genetic screens. For such applications, a barcode library would ideally have high diversity (many unique barcodes), non-destructive identification (repeated measurements in the same cells or population), and fast, inexpensive readout (many cells and conditions). Current nucleic acid barcoding methods generate high diversity but require destructive and slow/expensive readout, and current fluorescence barcoding methods are non-destructive, fast, and inexpensive to readout but lack high diversity. We recently proposed theory for how fluorescent protein combinations may generate a high-diversity barcode library with non-destructive, fast and inexpensive identification. Here, we presen

SUBMITTER: Lu X 

PROVIDER: S-EPMC11526929 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature