Modeling Glioma Intratumoral Heterogeneity with Primary Human Neural Stem and Progenitor Cells.

ABSTRACT: Glioblastoma multiforme (GBM) is a deadly form of glioma notable for its significant intratumoral heterogeneity, which is believed to drive therapy resistance. GBM has been observed to mimic a neural stem cell hierarchy reminiscent of normal brain development. However, it is still unclear how cell-of-origin shapes intratumoral heterogeneity. Here, we develop a model of glioma initiation using neural stem and progenitor cells (NSPCs) purified from fetal human brain tissue. We previously described a method to prospectively isolate and culture tripotent neural stem cells (NSCs), bipotent glial progenitor cells (GPCs), and unipotent oligodendrocyte precursor cells (OPCs). We transduced these isogenic lines with dominant-negative TP53^R175H and NF1 knockdown, a commonly-used genetic m

SUBMITTER: Gao D 

PROVIDER: S-EPMC11526988 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature