A Practical and Safe Model of Nitrogen Mustard Injury in Cornea.

ABSTRACT:

Purpose

Sulfur mustard (SM) is an alkylating agent used in warfare and terrorism that inflicts devastating ocular injuries. Although the clinical symptoms are well described, the underlying mechanisms are not fully understood, hindering the development of effective treatments. One major roadblock is the lack of a suitable model due to the extremely hazardous nature of SM, which requires strict safety measures. As a safe and practical alternative, we report a novel model that uses mechlorethamine (nitrogen mustard) gel, an FDA-approved topical chemotherapeutic administered by patients at home. Here we demonstrate its suitability to induce mustard corneal injury in any laboratory.

Methods

Ex vivo porcine corneas were injured with mechlorethamine gel. Hematoxylineosin st

SUBMITTER: Sandoval-Castellanos AM 

PROVIDER: S-EPMC11527019 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature