Correlating Disordered Activation Domain Ensembles with Gene Expression Levels.

ABSTRACT: Transcription factor proteins bind to specific DNA promoter sequences and initiate gene transcription. In eukaryotes, most transcription factors contain intrinsically disordered activation domains (ADs) that regulate their transcriptional activity. Like other disordered protein regions, ADs do not have a fixed three-dimensional structure and instead exist in an ensemble of conformations. Disordered ensembles contain sequence-encoded structural preferences which are often linked to their function. We hypothesize this link exists between the structural preferences of disordered AD ensembles and their ability to induce gene expression. To test this, we used FRET microscopy to measure the ensemble dimensions of two activation domains, HIF-1α and CITED2, in live cells, and correlate this struct

SUBMITTER: Flores E 

PROVIDER: S-EPMC11527027 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature