Reference Sequence Browser: An R application with a user-friendly GUI to rapidly query sequence databases.

ABSTRACT: Land managers, researchers, and regulators increasingly utilize environmental DNA (eDNA) techniques to monitor species richness, presence, and absence. In order to properly develop a biological assay for eDNA metabarcoding or quantitative PCR, scientists must be able to find not only reference sequences (previously identified sequences in a genomics database) that match their target taxa but also reference sequences that match non-target taxa. Determining which taxa have publicly available sequences in a time-efficient and accurate manner currently requires computational skills to search, manipulate, and parse multiple unconnected DNA sequence databases. Our team iteratively designed a Graphic User Interface (GUI) Shiny application called the Reference Sequence Browser (RSB) that provides

SUBMITTER: Ramesh S 

PROVIDER: S-EPMC11527216 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature