Modeling Platelet P2Y₁/₁₂ Pathway to Integrin Activation.

ABSTRACT: Through experimental studies, many details of the pathway of integrin αIIVβ3 activation by ADP during the platelet aggregation process have been mapped out. ADP binds to two separate G protein coupled receptors on platelet surfaces, leading to alterations in the regulation of the small GTPase RAP1. We seek to (1) gain insights into the relative contributions of both pathways to RAP1-mediated integrin activation and to (2) predict cell behavior in response to a continuous range of external agonist concentrations. To this end, we develop a dynamical systems model detailing the action of each protein in the two pathways up to the regulation of RAP1. We perform a parameter estimation using flow cytometry data to determine a number of unknown rate constants. We then validate with already publis

SUBMITTER: Patel KB 

PROVIDER: S-EPMC11527272 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature