Alanine Scanning to Define Membrane Protein-Lipid Interaction Sites Using Native Mass Spectrometry.

ABSTRACT: Lipids surrounding membrane proteins interact with different sites on the protein at varying specificities, ranging from highly specific to weak interactions. These interactions can modulate the structure, function, and stability of membrane proteins. Thus, to better understand membrane protein structure and function, it is important to identify the locations of lipid binding and the relative specificities of lipid binding at these sites. In our previous native mass spectrometry (MS) study, we developed a single and double mutant analysis approach to profile the contribution of specific residues toward lipid binding. Here, we extend this method by screening a broad range of mutants of AqpZ to identify specific lipid binding sites and by measuring binding of different lipid types to measure

SUBMITTER: Jayasekera HS 

PROVIDER: S-EPMC11527333 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature