Screening of Diabetes-Associated Autoantigens and Serum Antibody Profiles Using a Phage Display System.

ABSTRACT: Aims/Introduction: Phage display method is a crucial tool to find novel clinically valuable diabetes-associated autoantigens and identify known autoantigen epitopes that are associated with diabetes and could provide scientific support and guidance for the artificial construction and synthesis of Type I diabetes mellitus (T1DM) novel biomarkers. Materials and Methods: The phage display system was used for the "biopanning" of T1DM serum. Following the sequencing of the phage DNAs, the homologous sequences of the above fusion heptapeptide were further investigated by BLAST to track the origin of the polypeptide sequences. The antibody spectrum revealed new T1DM-associated epitopes and antibodies. Results: A total of 1200 phage DNA were sequenced and 9 conserved polypepti

SUBMITTER: Lin J 

PROVIDER: S-EPMC11527542 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature