Tailored biosynthesis of diosmin through reconstitution of the flavonoid pathway in <i>Nicotiana benthamiana</i>.

ABSTRACT: The flavonoid diosmin (diosmetin 7-O-rutinoside) is used as a therapeutic agent for disorders of the blood vessels such as hemorrhoids and varicose veins. Diosmin is commercially produced using semi-synthetic methods involving the oxidation of hesperidin, the most abundant flavonoid in citrus fruits. However, this method produces byproducts that are toxic to the environment, and new sustainable methods to produce diosmin are required. Here, we used a synthetic biology approach to produce diosmin without generating toxic byproducts through reconstitution of the diosmin biosynthetic pathway in Nicotiana benthamiana. We first established that N. benthamiana leaves co-infiltrated with all seven genes in the flavonoid biosynthesis pathway produced high levels of luteolin, a

SUBMITTER: Lee H 

PROVIDER: S-EPMC11527692 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature