Small-molecule properties define partitioning into biomolecular condensates.

ABSTRACT: Biomolecular condensates regulate cellular function by compartmentalizing molecules without a surrounding membrane. Condensate function arises from the specific exclusion or enrichment of molecules. Thus, understanding condensate composition is critical to characterizing condensate function. Whereas principles defining macromolecular composition have been described, understanding of small-molecule composition remains limited. Here we quantified the partitioning of ~1,700 biologically relevant small molecules into condensates composed of different macromolecules. Partitioning varied nearly a million-fold across compounds but was correlated among condensates, indicating that disparate condensates are physically similar. For one system, the enriched compounds did not generally bind macromolec

SUBMITTER: Ambadi Thody S 

PROVIDER: S-EPMC11527791 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature