Scalable production of uniform and mature organoids in a 3D geometrically-engineered permeable membrane.

ABSTRACT: The application of organoids has been limited by the lack of methods for producing uniformly mature organoids at scale. This study introduces an organoid culture platform, called UniMat, which addresses the challenges of uniformity and maturity simultaneously. UniMat is designed to not only ensure consistent organoid growth but also facilitate an unrestricted supply of soluble factors by a 3D geometrically-engineered, permeable membrane-based platform. Using UniMat, we demonstrate the scalable generation of kidney organoids with enhanced uniformity in both structure and function compared to conventional methods. Notably, kidney organoids within UniMat show improved maturation, showing increased expression of nephron transcripts, more in vivo-like cell-type balance, enhanced vascularization

SUBMITTER: Kim D 

PROVIDER: S-EPMC11528013 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature