Enzyme-encapsulated metal-organic framework ZIF-8-mediated biosensor for ultrasensitive detection of urinary prostatic exosomal protein using a glucose meter.

ABSTRACT: A highly sensitive and efficient home-use method is impressive and promising in the self-monitoring of chronic prostatitis (CP). Herein, we developed a glucose oxidase-zeolitic imidazolate framework-8-antibody composite (GOx@ZIF-8@Ab₂) to achieve highly sensitive point-of-care testing (POCT) of urinary prostatic exosomal protein (PSEP) by combining it with a personal glucometer (PGM). The developed GOx@ZIF-8@Ab₂ was prepared through a simple but effective biomineralization reaction and a strong binding affinity between Zn²⁺ and Fc region of the antibody. This bifunctional material GOx@ZIF-8@Ab₂ could not only specifically recognize the PSEP via immunoreaction but also wrap a large number of GOx molecules. Following the sandwich immunoreact

SUBMITTER: Lin M 

PROVIDER: S-EPMC11528330 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature