Nanomagnetic Guidance Shapes the Structure-Function Relationship of Developing Cortical Networks.

ABSTRACT: In this study, we implement large-scale nanomagnetic guidance on cortical neurons to guide dissociated neuronal networks during development. Cortical networks cultured over microelectrode arrays were exposed to functionalized magnetic nanoparticles, followed by magnetic field exposure to guide neurites over 14 days in vitro. Immunofluorescence of the axonal protein Tau revealed a greater number of neurites that were longer and aligned with the nanomagnetic force relative to nonguided networks. This was further confirmed through brightfield imaging on the microelectrode arrays during development. Spontaneous electrophysiological recordings revealed that the guided networks exhibited increased firing rates and frequency in force-aligned connectivity identified through Granger Causalit

SUBMITTER: Beck CL 

PROVIDER: S-EPMC11529602 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature