Global characterization of somatic mutations and DNA methylation changes during vegetative propagation in strawberries.

ABSTRACT: Somatic mutations arise and accumulate during tissue culture and vegetative propagation, potentially affecting various traits in horticultural crops, but their characteristics are still unclear. Here, somatic mutations in regenerated woodland strawberry derived from tissue culture of shoot tips under different conditions and 12 cultivated strawberry individuals are analyzed by whole genome sequencing. The mutation frequency of single nucleotide variants is significantly increased with increased hormone levels or prolonged culture time in the range of 3.3 × 10^-8-3.0 × 10^-6 mutations per site. CG methylation shows a stable reduction (0.71%-8.03%) in regenerated plants, and hypoCG-DMRs are more heritable after sexual reproduction. A high-quality haplotype-resolved genome

SUBMITTER: Hu S 

PROVIDER: S-EPMC11529994 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature