Essential magnetosome proteins MamI and MamL from magnetotactic bacteria interact in mammalian cells.

ABSTRACT: To detect cellular activities deep within the body using magnetic resonance platforms, magnetosomes are the ideal model of genetically-encoded nanoparticles. These membrane-bound iron biominerals produced by magnetotactic bacteria are highly regulated by approximately 30 genes; however, the number of magnetosome genes that are essential and/or constitute the root structure upon which biominerals form is largely undefined. To examine the possibility that key magnetosome genes may interact in a foreign environment, we expressed mamI and mamL as fluorescent fusion proteins in mammalian cells. Localization and potential protein-protein interaction(s) were investigated using confocal microscopy and fluorescence correlation spectroscopy (FCS). Enhanced green fluorescent protein (EGFP)-MamI and t

SUBMITTER: Sun Q 

PROVIDER: S-EPMC11530650 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature