A fluorescence lifetime-based novel method for accurate lipid quantification of BODIPY vital-stained C. elegans.

ABSTRACT: Lipid droplets (LDs) are organelles associated with lipid storage and energy metabolism, thus, their morphology and quantity are of significant research interest. While commercially available BODIPY dye effectively labels LDs in various cell types, it also labels lysosome-related organelles (LROs) in C. elegans, leading to non-specific LD quantification. Here, we report that the fluorescent signals of BODIPY exhibit distinct fluorescence lifetime patterns for LROs and LDs, which can be captured, visualized, and filtered by fluorescence lifetime imaging microscopy. Furthermore, we proposed and validated a method based on fluorescence lifetime that can improve the accuracy of fat storage quantification in BODIPY vital-staining worms, which holds broad applications, including rapid and accura

SUBMITTER: Xu C 

PROVIDER: S-EPMC11530801 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature