TNFα has differential effects on the transcriptome profile of selected populations in murine cartilage.

ABSTRACT:

Objective

To further our understanding of the role of tumor necrosis factor (TNF)α on the inflammatory response in chondrocytes.

Design

We explored the effects of TNFα on the transcriptome of epiphyseal chondrocytes from newborn C57BL/6 mice at the total and single cell (sc) resolution.

Results

Gene set enrichment analysis of total RNA-Seq from TNFα-treated chondrocytes revealed enhanced response to biotic stimulus, defense and immune response and cytokine signaling and suppressed cartilage and skeletal morphogenesis and development. scRNA-Seq analyzed 14,239 ​cells and 24,320 genes and distinguished 16 ​cell clusters. The more prevalent ones were constituted by limb bud and chondrogenic cells and fibroblasts comprising ∼73 ​% of the cell population. Genes expressed b

SUBMITTER: Canalis E 

PROVIDER: S-EPMC11530803 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature