NLC Delivery of EGFP Plasmid to TM4 Cell Nuclei for Targeted Gene Therapy.

ABSTRACT:

Purpose

This study evaluated whether a nanostructured lipid carrier (NLC) delivery system could safely and accurately deliver nucleic acids to the cell nucleus using the enhanced green fluorescent protein (EGFP)-C1 plasmid model.

Methods

The NLC was formulated using the emulsification method and equipped for cationic lipid-mediated transfection with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), which interacts electrostatically with nucleic acid. The NLC attributes, including size, polydispersity index, and zeta potential, were assessed by dynamic light scattering (DLS). The morphological structure was analyzed using transmission electron microscopy. Entrapment efficiency was evaluated by a direct method. Cellular uptake mechanisms of pEGFP-C1-NLC and the ability of pEGF

SUBMITTER: Jummah N 

PROVIDER: S-EPMC11530889 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature