Association of tumour mutation burden with prognosis and its clinical significance in stage III gastric cancer.

ABSTRACT:

Introduction

To explore the correlation between the tumour mutation burden (TMB) and prognosis and its clinical significance among patients with stage III gastric cancer (GC).

Methods

Patients with stage III GC were divided into a high TMB and low TMB group in both a study cohort of 38 patients and the Cancer Genome Atlas (TCGA) cohort of 173 patients. In the study cohort, next-generation sequencing was used to detect mutated GC genes and obtain TMB data. In the TCGA cohort, gene set enrichment analysis was performed, and the relationship between TMB, prognosis and clinicopathologic factors was analysed. Western blot and quantitative real-time polymerase chain reaction were used to detect the expression levels of both proteins and genes. Cell viability was measured using met

SUBMITTER: Han YL 

PROVIDER: S-EPMC11530966 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature