13 C-MFA helps to identify metabolic bottlenecks for improving malic acid production in Myceliophthora thermophila.

ABSTRACT:

Background

Myceliophthora thermophila has been engineered as a significant cell factory for malic acid production, yet strategies to further enhance production remain unclear and lack rational guidance. ¹³C-MFA (¹³C metabolic flux analysis) offers a means to analyze cellular metabolic mechanisms and pinpoint critical nodes for improving product synthesis. Here, we employed ¹³C-MFA to investigate the metabolic flux distribution of a high-malic acid-producing strain of M. thermophila and attempted to decipher the crucial bottlenecks in the metabolic pathways.

Results

Compared with the wild-type strain, the high-Malic acid-producing strain M. thermophila JG207 exhibited greater glucose uptake and carbon dioxide evolution rates but lower oxygen u

SUBMITTER: Jiang J 

PROVIDER: S-EPMC11531171 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature