Therapeutic potential of omaveloxolone in counteracting muscle atrophy post-denervation: a multi-omics approach.

ABSTRACT:

Background

Muscle atrophy caused by denervation is common in neuromuscular diseases, leading to loss of muscle mass and function. However, a comprehensive understanding of the overall molecular network changes during muscle denervation atrophy is still deficient, hindering the development of effective treatments.

Method

In this study, a sciatic nerve transection model was employed in male C57BL/6 J mice to induce muscle denervation atrophy. Gastrocnemius muscles were harvested at 3 days, 2 weeks, and 4 weeks post-denervation for transcriptomic and proteomic analysis. An integrative multi-omics approach was utilized to identify key genes essential for disease progression. Targeted proteomics using PRM was then employed to validate the differential expression of central genes.

SUBMITTER: Wang S 

PROVIDER: S-EPMC11531194 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature