Single intra-articular injection of human synovial membrane MSC from grade IV knee osteoarthritis patient improve cartilage repair in OA rat model.

ABSTRACT:

Aim

This study aims to assess the effectiveness of therapy of human synovial membrane-derived MSCs (SM-MSC) from OA grade IV patients in treating knee OA.

Methods

SM-MSC were isolated from patients undergoing total knee replacement surgery, cultured to the fourth passage, and characterized using flow cytometry. Differentiation potential was assessed through lineage-specific staining. Osteoarthritis was induced in 24 Wistar rats via monosodium iodoacetate (MIA). The rats were divided into three groups: negative control, OA control, and OA treated with SM-MSC. Radiological, histopathological, and molecular analyses were conducted to evaluate cartilage repair and gene expression.

Results

Flow cytometry confirmed the MSC phenotype of SM-MSC, and successful differentiation

SUBMITTER: Rahmadian R 

PROVIDER: S-EPMC11531201 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature