Mechanism of CXCL8 regulation of methionine metabolism to promote angiogenesis in gliomas.

ABSTRACT:

Background

Gliomas are the most common malignant brain tumors characterized by angiogenesis and invasive growth. A detailed understanding of its molecular characteristics could provide potential therapeutic targets. In the present study, we sought to explore the key gene CXCL8 in methionine metabolism in gliomas and its potential role in angiogenesis.

Methods

U251 glioma cells were divided into control and methionine-restriction tolerant (constructed with 1/4 of the standard level of methionine in the culture medium) groups for transcriptome and metabolome analysis. To confirm the functions and mechanism of CXCL8 in glioma, heat map, volcano map, Go enrichment, gene set enrichment analysis (GSEA), protein-protein interaction network analysis, RT-PCR, western blotting assays,

SUBMITTER: Chang J 

PROVIDER: S-EPMC11531453 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature