Antibacterial peptides from black soldier fly (Hermetia illucens) larvae: mode of action and characterization.

ABSTRACT: Antibacterial peptides from black soldier fly larvae extract were prepared using Flash column chromatography. Three out of five fractions (F₂, F₃ and F₄) showed antibacterial activity against Listeria monocytogenes DMST 17303 with a minimum inhibitory concentration (MIC) of 1 mM, followed by Salmonella enterica Enteritidis DMST 15679 and Escherichia coli O157:H7 DMST 12743 with a MIC ranging from 4 to 8 mM. Due to the higher yield, F₂ and F₃ were further analyzed on their mode of action against L. monocytogenes DMST 17303. Both fractions, particularly F₂, exerted antibacterial activity through inducing bacterial cell membrane disintegration and interaction with intracellular compounds including fatty acids, proteins, and nuc

SUBMITTER: Pimchan T 

PROVIDER: S-EPMC11531597 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature