Embryotrophic effect of exogenous protein contained adipose-derived stem cell extracellular vesicles.

ABSTRACT:

Background

Extracellular vesicles (EVs) regulate cell metabolism and various biological processes by delivering specific proteins and nucleic acids to surrounding cells. We aimed to investigate the effects of the cargo contained in EVs derived from adipose-derived stem cells (ASCs) on the porcine embryonic development.

Methods

ASCs were isolated from porcine adipose tissue and characterized using ASC-specific markers via flow cytometry. EVs were subsequently extracted from the conditioned media of the established ASCs. These EVs were added to the in vitro culture environment of porcine embryos to observe qualitative improvements in embryonic development. Furthermore, the proteins within the EVs were analyzed to investigate the underlying mechanisms.

Results

We observe

SUBMITTER: Bang S 

PROVIDER: S-EPMC11531693 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature