Identification of TPI1 As a potential therapeutic target in pancreatic cancer with dependency of TP53 mutation using multi-omics analysis.

ABSTRACT: Mutations of KRAS, CDKN2A, TP53, and SMAD4 are the four major driver genes for pancreatic ductal adenocarcinoma (PDAC), of which mutations of KRAS and TP53 are the most frequently recognized. However, molecular-targeted therapies for mutations of KRAS and TP53 have not yet been developed. To identify novel molecular targets, we newly established organoids with the Kras mutation (Kras^muOR) and Trp53 loss of function using Cre transduction and CRISPR/Cas9 (Kras^mu/p53^muOR) from murine epithelia of the pancreatic duct in Kras^LSL-G12D mice, and then analyzed the proteomic and metabolomic profiles in both organoids by mass spectrometry. Hyperfunction of the glycolysis pathway was recognized in Kras^mu/p53^muOR compared with Kras

SUBMITTER: Toyoda T 

PROVIDER: S-EPMC11531968 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature