MitoTracker Red for isolation of zone-specific hepatocytes and characterization of hepatic sublobular metabolism.

ABSTRACT:

Background

The liver lobule is divided into three zones or regions: periportal (PP or Zone 1) that is highly oxidative and active in ureagenesis, pericentral (PC or Zone 3) that is more glycolytic, and midzonal (MZ or Zone 2) with intermediate characteristics.

Aim

Our goal was to isolate and metabolically characterize hepatocytes from specific sublobular zones.

Methods

Mice were administered rhodamine123 (Rh123) or MitoTracker Red (MTR) prior to intravital imaging, liver fixation, or hepatocyte isolation. After in vivo MTR, hepatocytes were isolated and sorted based on MTR fluorescence intensity. Alternatively, E-cadherin (Ecad) and cytochrome P450 2E1 (CYP2E1) immunolabeling was performed in fixed liver slices. Ecad and CYP2E1 gene expression in sorted hepatocytes wa

SUBMITTER: Savoca M 

PROVIDER: S-EPMC11532002 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature