Pancreatic β-cells package double C2-like domain beta protein into extracellular vesicles via tandem C2 domains.

ABSTRACT:

Introduction

Double C2-like domain beta (DOC2B) is a vesicle priming protein critical for glucose-stimulated insulin secretion in β-cells. Individuals with type 1 diabetes (T1D) have lower levels of DOC2B in their residual functional β-cell mass and platelets, a phenotype also observed in a mouse model of T1D. Thus, DOC2B levels could provide important information on β-cell dys(function).

Objective

Our objective was to evaluate the DOC2B secretome of β-cells. In addition to soluble extracellular protein, we assessed DOC2B localized within membrane-delimited nanoparticles - extracellular vesicles (EVs). Moreover, in rat clonal β-cells, we probed domains required for DOC2B sorting into EVs.

Method

Using Single Extracellular VEsicle Nanoscopy, we quantified EVs derived f

SUBMITTER: Esparza D 

PROVIDER: S-EPMC11532064 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature