AAVolve: Concatenated long-read deep sequencing enables whole capsid tracking during shuffled AAV library selection.

ABSTRACT: Gene therapies using recombinant adeno-associated virus (AAV) vectors have demonstrated considerable clinical success in the treatment of genetic disorders. Improved vectors with favorable tropism profiles, decreased immunogenicity, and enhanced manufacturability are poised to further improve the state of gene therapies. Such vectors can be identified through directed evolution, a process of subjecting a diverse capsid library to a selection pressure to identify individual variants with a desired trait. Currently, libraries that involve changes distributed throughout the AAV capsid coding region, such as DNA family shuffled libraries, are largely characterized using low-throughput Sanger sequencing of individual clones. However, improvements in long-read sequencing technologies have increa

SUBMITTER: Scott S 

PROVIDER: S-EPMC11532298 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature