Decoding the epigenetics and chromatin loop dynamics of androgen receptor-mediated transcription.

ABSTRACT: Androgen receptor (AR)-mediated transcription plays a critical role in development and prostate cancer growth. AR drives gene expression by binding to thousands of cis-regulatory elements (CRE) that loop to hundreds of target promoters. With multiple CREs interacting with a single promoter, it remains unclear how individual AR bound CREs contribute to gene expression. To characterize the involvement of these CREs, we investigate the AR-driven epigenetic and chromosomal chromatin looping changes by generating a kinetic multi-omic dataset comprised of steady-state mRNA, chromatin accessibility, transcription factor binding, histone modifications, chromatin looping, and nascent RNA. Using an integrated regulatory network, we find that AR binding induces sequential changes in the epigenetic fe

SUBMITTER: Altıntas UB 

PROVIDER: S-EPMC11532539 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature