METTL16 inhibits differentiation and promotes proliferation and slow myofibers formation in chicken myoblasts.

ABSTRACT: N6-methyladenosine (m6A) plays a crucial regulatory role in muscle growth and development. In our previous studies, we identified a m6A methyltransferase, Methyltransferase like 16 (METTL16), which is associated with chicken muscle development and muscle fiber type conversion. To further understand the regulatory role of METTL16 in chicken muscle function, we analyzed its expression in muscle tissues with different myofiber type compositions and in chicken primary myoblasts (CPMs) at various stages. We also manipulated METTL16 expression in CPMs to examine its effects on cell proliferation, differentiation, muscle fiber type formation, and global m6A RNA methylation status. Our results showed that METTL16 expression increased during myoblast proliferation and gradually decreased in the lat

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11532546 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature