Mechanical Microvibration Device Enhancing Immunohistochemistry Efficiency.

ABSTRACT: Immunohistochemistry (IHC) is a widely used technique in diagnostic pathology and biomedical research, but there is still a need to shorten the operation process and reduce the cost of antibodies. This study aims to assess a novel IHC technique that incorporates mechanical microvibration (MMV) to expedite the process, reduce antibody consumption, and enhance staining quality. MMV was generated using coin vibration motors attached to glass slides mounted with consecutive tissue sections. Multiple antibodies targeting various antigens were used to stain cancerous and normal tissues, with and without microvibration. Various parameters were tested, including incubation durations, temperatures, and antibody dilutions. The novel method showed the potential to achieve comparable or superior outco

SUBMITTER: Zhang W 

PROVIDER: S-EPMC11532639 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature