Minimizing ice contamination during specimen preparation for cryo-soft X-ray tomography and cryo-electron tomography.

ABSTRACT: Cryo-soft X-ray tomography (cryo-SXT) is a newly developed technique for imaging 3D whole cells in nearly native states. Cryo-SXT users require the preparation of numerous cryo-sample grids to use the allocated beamtime to study cellular phenomena under various conditions. Therefore, it is important to promptly prepare cryo-sample grids as efficiently and carefully as possible to minimize ice contamination on the frozen sample grid. In this study, we designed a cryo-multi-grid-box storage system, which includes a shell, funnel holder, and multi-grid-box container. Our system not only increases the number of cryo-sample grids that can be temporarily stored but also reduces the frequency of cryo grid-box container transfers, thus decreasing the probability of forming ice on the grid. We have

SUBMITTER: Hsieh CC 

PROVIDER: S-EPMC11532778 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature