Kinetic and catalytic mechanisms of the methionine-derived glucosinolate biosynthesis enzyme methylthioalkylmalate synthase.

ABSTRACT: In Brassica plants, methionine-derived aliphatic glucosinolates are chemically diverse natural products that serve as plant defense compounds, as well as molecules with dietary health-promoting effects. During their biosynthesis, methylthioalkylmalate synthase (MAMS) catalyzes the elongation reaction of the aliphatic chain. The MAMS-catalyzed condensation of 4-methylthio-2-oxobutanoic acid and acetyl-CoA generates a 2-malate derivative that either enters the pathway for the synthesis of C₃-glucosinolates or undergoes additional extension reactions, which lead to C₄- to C₉-glucosinolates. Recent determination of the x-ray crystal structure of MAMS from Brassica juncea (Indian mustard) provided insight on the molecular evolution of MAMS, especially substrate

SUBMITTER: Kitainda V 

PROVIDER: S-EPMC11532901 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature