Pupylation-Based Proximity-Tagging of FERONIA-Interacting Proteins in Arabidopsis.

ABSTRACT: The plasma membrane-localized receptor kinase FERONIA (FER) plays critical roles in a remarkable variety of biological processes throughout the life cycle of Arabidopsis thaliana. Revealing the molecular connections of FER that underlie these processes starts with identifying the proteins that interact with FER. We applied pupylation-based interaction tagging (PUP-IT) to survey cellular proteins in proximity to FER, encompassing weak and transient interactions that can be difficult to capture for membrane proteins. We reproducibly identified 581, 115, and 736 specific FER-interacting protein candidates in protoplasts, seedlings, and flowers, respectively. We also confirmed 14 previously characterized FER-interacting proteins. Protoplast transient gene expression expedited the testing of ne

SUBMITTER: Lin Z 

PROVIDER: S-EPMC11532908 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature