MicroRNA-3061 downregulates the expression of PAX7/Wnt/Ca²⁺ signalling axis genes to induce premature ovarian failure in mice.

ABSTRACT: The in-depth mechanisms of microRNA regulation of premature ovarian failure (POF) remain unclear. Crispr-cas9 technology was used to construct transgenic mice. The qPCR and Western blot was used to detect the expression level of genes. H&E staining were used to detect ovarian pathological phenotypes. We found that the expression levels of microRNA-3061 were significantly higher in ovarian granulosa cells (OGCs) of POF mouse models than in controls. The miR-3061^+/-/AMH-Cre^+/- transgenic mice manifested symptoms of POF. RNA-Seq and luciferase reporter assay confirmed that the PAX7 was one of the target genes negatively regulated by microRNA-3061 (miR-3061-5p). Moreover, PAX7 mediated the expression of non-canonical Wnt/Ca²⁺ signalling pathway by binding to th

SUBMITTER: Liu T 

PROVIDER: S-EPMC11533063 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature