Combined lineage tracing and scRNA-seq reveal the activation of Sox9⁺ cells in renal regeneration with PGE₂ treatment.

ABSTRACT: Uncovering mechanisms of endogenous regeneration and repair through resident stem cell activation will allow us to develop specific therapies for injuries and diseases by targeting resident stem cell lineages. Sox9⁺ stem cells have been reported to play an essential role in acute kidney injury (AKI). However, a complete view of the Sox9⁺ lineage was not well investigated to accurately elucidate the functional end state and the choice of cell fate during tissue repair after AKI. To identify the mechanisms of fate determination of Sox9⁺ stem cells, we set up an AKI model with prostaglandin E2 (PGE₂) treatment in a Sox9 lineage tracing mouse model. Single-cell RNA sequencing (scRNA-seq) was performed to analyse the transcriptomic profile of the Sox9

SUBMITTER: Chen S 

PROVIDER: S-EPMC11533080 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature